レポーターアッセイ試薬選択

レポーターアッセイ試薬一覧をご覧いただけます。
Nano-Glo・Dual-Luc・Dual-Glo・ONE-Glo・Steady-Glo・Bright-Glo等

Choosing a Luciferase Reporter Assay A reporter assay comparison guide. This guide will help you learn about reporter assays and find the one best suited for your needs. Visit the Promega catalog listing to view ordering information for the Reporter Assays featured here. Take me to Reporter Assays Reporter Genes and Assay Chemistries When creating a luciferase reporter assay, there are two important elements to consider: the luciferase reporter protein itself and the assay chemistry used to detect reporter activity. The characteristics of these two components together contribute to the overall performance of the assay. By selecting the reporter/detection solution that is optimal for your experimental goals, you can customize your luciferase reporter assay to create the best solution for your research. Promega offers a choice of 3 different luciferase reporters: NanoLuc® Luciferase(Nluc,19kDa), Renilla Luciferase (Rluc, 36kDa) and Firefly Luciferase (Fluc, 61kDa) which vary in size, brightness, and protein half-life. Luciferase Reporters Reporter gene names Firefly Luciferase Firefly Luciferase descriptive text luc, luc+, luc2, luc2P, luc2CP Renilla Luciferase Rluc, hRluc, hRlucP, hRlucCP NanoLuc Luciferase Nluc, NlucP, secNluc Detection Reagent Considerations Multiple assay detection reagents are also available for each reporter. Key considerations for selecting the optimal assay reagent include: Signal intensity and overall dynamic range needed for the assay. Signal stability, or half-life, which will impact your processing workflow. Processing steps required. Non-homogenous assays require a separate lysate creation step prior to reagent addition. Homogenous assay reagents are added directly to the cells in culture eliminating sample pre-processing. Lytic or live-cell reporter detection Single or dual-reporter detection Luciferase Signal Strength and Stability Dual-Luciferase® Assay Options Firefly Luciferase Assay Options Here, we compared luminescence signals from HEK293 cells transfected with a 1:1:8 ratio of either TK-Rluc (Renilla):TK-Fluc (firefly):carrier DNA or TK-Nluc (NanoLuc):TK-Fluc:carrier DNA and assayed with NanoDLR™, DLR™ or Dual-Glo® Luciferase Assay Systems as indicated. The NanoDLR(TM) and Dual-Glo(R) reagents are homogenous assay systems with increased signal stability. The DLR(TM) reagent is a non-homogenous assay with a flash signal that decays rapidly. Here we demonstrate luminescence signal over time from a dilution of QuantiLum® Recombinant Luciferase assayed with various firefly luciferase detection reagents as indicated. The Luciferase Assay System is a non-homogenous reagent that provides the brightest initial luminescence with flash kinetics that has rapid signal decay. The Bright-Glo™, ONE-Glo™, ONE-Glo™ EX, and Steady-Glo® systems are homogenous reagents that show progressively decreasing levels of initial brightness with respective increases in signal half-life. Compare Luciferase Assay Characteristics Luciferase Detected Assay Reagent Ideal For Signal Half-Life Sensitivity Number of Steps Live Cell Assay? Injectors Needed? NanoLuc, Firefly Nano-Glo® Dual-Luciferase® Reporter Assay System (NanoDLR) Homogenous Fluc/Nluc dual-reporter detection with flexibility in choice of primary reporter. Provides highest sensitivity for both reporters when a stable signal is required and highest sensitivity option when Nluc is used as the primary reporter. Ideal for low to high-throughput processing when 2 primary reporters or internal control is needed. 2 hours each +++++ (Nluc) ++ (Fluc) 2 (lysate optional) No Optional Firefly, Renilla Dual-Luciferase® Reporter Assay System (DLR) Non-homogenous Fluc/Rluc dual-reporter detection. Requires lysate creation and 2 injectors for delivery. Ideal for small sample numbers when maximum Fluc sensitivity and internal control are needed. 10 minutes, 2 minutes ++++ (Fluc) ++++ (Rluc) 3 No Yes 2 injectors Firefly, Renilla Dual-Glo® Luciferase Assay System Homogenous Fluc/Rluc dual-reporter detection with reduced sensitivity and stable signal. Ideal for high-throughput processing of Fluc reporter when Rluc is used as the internal control. 2 hours each + (Fluc/Rluc) 2 No No NanoLuc Nano-Glo® Luciferase Assay System Homogenous Nluc detection with bright, stable signal. Ideal for low to high-throughput processing when maximum sensitivity is required. 2 hours +++++ 1 No, unless used with secNluc No NanoLuc Nano-Glo® Live Cell Assay System Live cell Nluc detection with brightest signal. Ideal for single timepoint analysis when high sensitivity is needed in a non-lytic assay. 30 minutes ++++ 1 Yes No NanoLuc Nano-Glo® Vivizine™ Live Cell Substrate Live cell Nluc detection with intermediate signal and stability. Ideal for kinetic analysis lasting multiple hours. 12 hours +++ 1 Yes No NanoLuc Nano-Glo® Endurazine™ Live Cell Substrate Live cell Nluc detection with most stable signal. Ideal for multi-day kinetic analysis. Up to 72 hours ++ 1 Yes No Firefly Luciferase Assay System Non-homogenous Fluc detection requiring lysate creation and injector delivery. Ideal for small sample numbers when maximum Fluc sensitivity is needed. 10 minutes ++++ 2 No Yes Firefly Bright-Glo® Luciferase Assay System Homogenous Fluc detection offering the brightest signal and shortest half-life. Ideal for high-throughput processing when high sensitivity is required. 30 minutes +++ 1 No No Firefly ONE-Glo™ Luciferase Assay System Homogenous Fluc detection with moderate signal and half-life. Ideal for high- or ultrahigh-throughput processing. 45 minutes ++ 1 No No Firefly ONE-Glo™ Ex Luciferase Assay System Homogenous Fluc detection with moderate signal and half-life and improved storage stability. Ideal for high- or ultrahigh-throughput processing and repeat use. Also used to detect Fluc in NanoDLR allowing consistent use between single and dual assays. 2 hours ++ 1 No No Firefly Steady-Glo® Luciferase Assay System Homogenous Fluc detection with maximum signal stability and reduced signal. Ideal for high-throughput applications when extended luminescence is required. 5 hours + 1 No No Renilla Renilla Luciferase Assay System Non-homogenous Rluc detection requiring lysate creation and injector delivery. Ideal for small sample numbers when maximum Rluc sensitivity is needed. 2 minutes ++++ 2 No Yes Renilla Renilla-Glo Luciferase Assay System Homogenous Rluc detection with increased signal stability. Ideal for high-throughput processing. 40+ minutes ++ 1 No No Renilla ViviRen™ Live Cell Substrate Live cell Rluc detection with highest signal. Ideal for single time point or short non-lytic analysis when highest sensitivity is required. 10 minutes +++ 1 Yes No Renilla EnduRen™ Live Cell Substrate Live cell Rluc detection with greatest stability. Ideal for extended kinetic analysis. Up to 24 hours + 1 Yes No Learn More About Bioluminescent Reporter Assay Design This two-part series begins by walking through the basic considerations for choosing the optimal experimental reporter. In part two, Assay Normalization Options”, experimental design and data analysis methods are discussed. Choosing a Reporter Assay Normalization Related Resources webinare-luciferase Guide: Reporter Genes and their Applications webiar-related-resource Webinar: Understanding Reporter Assay Design blog-resources Blog: Tips for Performing Dual-Reporter Assays dual-reporter-assay-resource Blog: How Dual-Reporter Assays Can Save Your Data blog-when-to-inject Blog: To inject or not inject? infograph-resurce Infographic: How to Choose Primary and Control Reporters in the NanoDLR Assay プロメガクラブに入ろう！ 会員だけの特別サービス、イベントなどの特典が盛りだくさん！！ 新規入会 ご質問はこちら 連絡先情報 技術的質問・在庫照会 TEL 03 3669 7981 問合せは、問合せフォーム よりお願いします。 ※販売店様専用問合せサイトはこちら 製品関連情報 製品情報 アプリケーション 製品カスタマイズ 技術資料 プロメガについて 会社情報 ISO 認証 企業責任 プレスリリース 採用情報 Facebook Twitter LinkedIn © 2021 Promega Corporation, an affiliate of Promega KK. All rights reserved. Legal and Trademarks Privacy Policy Terms and Conditions Trademarks 日本語 – Japan