Protocols and Applications Guide（オンライン版）
分子生物学全般
Protocols and Applications Guide（オンライン版）
核酸の精製、修飾、シークエンシング、発現など分子生物学の実験手法を始め、細胞を用いたシグナル伝達・増殖に関わる情報も網羅したプロトコル集。
オンライン版になりました。
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Product and Technology Guides Learn more about basic and advanced methods for cell biology, nucleic acid analysis, protein analysis and basic research in these educational resources. Each guide provides an overview of the basic science behind the methods and techniques described, as well as links to products and other helpful resources. Cell Biology Nucleic Acid Analysis Lab Equipment and Supplies Protein Analysis Cell Biology Introduction to 3D Cell Culture 3D cell cultures are increasingly being used for basic research and drug discovery. Here you’ll learn what 3D cell cultures are, why and how they are being used, and how to validate assays that work with 3D models. ATP アッセイ ATPアッセイの測定原理および正しいアッセイの選び方 Cell Line Authentication Resources Misidentified cell lines can lead to lost resources, time and effort. Explore why cell line authentication is important and learn how to confirm the identity of your cells. Choosing a Luciferase Reporter Assay A comparison guide to help you select the right luciferase assay for your needs. Interpreting Multiplex Cell Health Data The CellTox Green Cytotoxicity Assay can be used with other cell health assays to provide richer information about why and how cells die. This interactive guide teaches you how to interpret the results from these experiments. Live-Cell Kinetic Assays for Time Course Analysis Learn about simplified cell-based assays that allow you to repeatedly measure one sample well over time. Assays for Biochemical Targets This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest. Cell Imaging An overview of methods used for imaging studies in cell biology. Reporter Genes and their Applications How to use bioluminescent reporter genes to study eukaryotic gene expression and cellular events. Apoptosis An introduction to apoptotic cell death, including methods to confirm apoptosis. Cell Viability Assay Guide How to choose the right cell viability assay for your needs. Transfection A guide to transfection including protocols and optimization tips. Nucleic Acid Analysis Subcloning Guide A guide to the fundamentals of molecular cloning, including restriction digestion, DNA ligation, vector dephosphorylation, and bacterial transformation. Restriction Enzyme Resource Guide Restriction enzymes 101, including reference information and links to restriction digest tools. DNA Purification An overview of DNA extraction methods for manual and automated purification needs. Epigenetics An introduction to the study of epigenetic changes in gene expression. PCR Amplification An overview of methods for PCR, RT-PCR and qPCR. Lab Equipment and Supplies Buffers for Biochemical Reactions All you need to know about preparing buffers. Fluorophores A guide to Promega Fluorometers and Fluorescence Modules or Optical Kits. Protein Analysis Protease Digestion for Mass Spectrometry A collection of protocols for in-gel and in-solution protein digestion with trypsin, trypsin/lysc or alternative proteases. Protein Expression An introduction to in vitro transcription and translation methods. Protein Purification An overview of common methods for protein purification. A Proteomics Product Guide An overview of products used for protein expression, purification and analysis.