NanoLuc® Luciferase Technology

サイテーション（PPI）

NanoBRET

※ 論文タイトルをクリックするとPubMedに移動します

タイトル	著者	ジャーナル名
Biochemical analysis of Rabin8, the guanine nucleotide exchange factor for Rab8.	Wu B, Wang J, Zhao Y, Guo W.	Methods Cell Biol. 2015;130:59-68.
Activation of Rab8 guanine nucleotide exchange factor Rabin8 by ERK1/2 in response to EGF signaling.	Wang J, Ren J, Wu B, Feng S, Cai G, Tuluc F, Peränen J, Guo W.	Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):148-53.
NanoBRET–A Novel BRET Platform for the Analysis of Protein-Protein Interactions.	Machleidt T, Woodroofe CC, Schwinn MK, Méndez J, Robers MB, Zimmerman K, Otto P, Daniels DL, Kirkland TA, Wood KV.	ACS Chem Biol. 2015 Aug 21;10(8):1797-804.
EZH2 inhibitor efficacy in non-Hodgkin’s lymphoma does not require suppression of H3K27 monomethylation.	Bradley WD, Arora S, Busby J, Balasubramanian S, Gehling VS, Nasveschuk CG, Vaswani RG, Yuan CC, Hatton C, Zhao F, Williamson KE, Iyer P, Méndez J, Campbell R, Cantone N, Garapaty-Rao S, Audia JE, Cook AS, Dakin LA, Albrecht BK, Harmange JC, Daniels DL, Cummings RT, Bryant BM, Normant E, Trojer P.	Chem Biol. 2014 Nov 20;21(11):1463-75.
9H-purine scaffold reveals induced-fit pocket plasticity of the BRD9 bromodomain.	Picaud S, Strocchia M, Terracciano S, Lauro G, Mendez J, Daniels DL, Riccio R, Bifulco G, Bruno I, Filippakopoulos P.	J Med Chem. 2015 Mar 26;58(6):2718-36.
Target engagement and drug residence time can be observed in living cells with BRET.	Robers MB, Dart ML, Woodroofe CC, Zimprich CA, Kirkland TA, Machleidt T, Kupcho KR, Levin S, Hartnett JR, Zimmerman K, Niles AL, Ohana RF, Daniels DL, Slater M, Wood MG, Cong M, Cheng YQ, Wood KV.	Nat Commun. 2015 Dec 3;6:10091.
Fluorescence- and bioluminescence-based approaches to study GPCR ligand binding.	Stoddart LA, White CW, Nguyen K, Hill SJ, Pfleger KD.	Br J Pharmacol. 2016 Oct;173(20):3028-37.
Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma.	Conery AR, Centore RC, Neiss A, Keller PJ, Joshi S, Spillane KL, Sandy P, Hatton C, Pardo E, Zawadzke L, Bommi-Reddy A, Gascoigne KE, Bryant BM, Mertz JA, Sims RJ.	Elife. 2016 Jan 5;5. pii: e10483.
Deciphering the Cellular Targets of Bioactive Compounds Using a Chloroalkane Capture Tag.	Ohana RF, Kirkland TA, Woodroofe CC, Levin S, Uyeda HT, Otto P, Hurst R, Robers MB, Zimmerman K, Encell LP, Wood KV.	ACS Chem Biol. 2015 Oct 16;10(10):2316-24.
LP99: Discovery and Synthesis of the First Selective BRD7/9 Bromodomain Inhibitor.	Clark PG, Vieira LC, Tallant C, Fedorov O, Singleton DC, Rogers CM, Monteiro OP, Bennett JM, Baronio R, Müller S, Daniels DL, Méndez J, Knapp S, Brennan PE, Dixon DJ.	Angew Chem Weinheim Bergstr Ger. 2015 May 18;127(21):6315-6319.
Enabling systematic interrogation of protein-protein interactions in live cells with a versatile ultra-high-throughput biosensor platform.	Mo XL, Luo Y, Ivanov AA, Su R, Havel JJ, Li Z, Khuri FR, Du Y, Fu H.	J Mol Cell Biol. 2016 Jun;8(3):271-81.
Generation of a Selective Small Molecule Inhibitor of the CBP/p300 Bromodomain for Leukemia Therapy.	Picaud S, Fedorov O, Thanasopoulou A, Leonards K, Jones K, Meier J, Olzscha H, Monteiro O, Martin S, Philpott M, Tumber A, Filippakopoulos P, Yapp C, Wells C, Che KH, Bannister A, Robson S, Kumar U, Parr N, Lee K, Lugo D, Jeffrey P, Taylor S, Vecellio ML, Bountra C, Brennan PE, O’Mahony A, Velichko S, Müller S, Hay D, Daniels DL, Urh M, La Thangue NB, Kouzarides T, Prinjha R, Schwaller J, Knapp S.	Cancer Res. 2015 Dec 1;75(23):5106-5119.
Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes.	McGrath JP, Williamson KE, Balasubramanian S, Odate S, Arora S, Hatton C, Edwards TM, O’Brien T, Magnuson S, Stokoe D, Daniels DL, Bryant BM, Trojer P.	Cancer Res. 2016 Apr 1;76(7):1975-88.
Inhibition of the p53/hDM2 protein-protein interaction by cyclometallated iridium(III) compounds.	Liu LJ, He B, Miles JA, Wang W, Mao Z, Che WI, Lu JJ, Chen XP, Wilson AJ, Ma DL, Leung CH.	Oncotarget. 2016 Mar 22;7(12):13965-75.
Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone.	Arts R, den Hartog I, Zijlema SE, Thijssen V, van der Beelen SH, Merkx M.	Anal Chem. 2016 Apr 19;88(8):4525-32.
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.	Ghosh S, Taylor A, Chin M, Huang HR, Conery AR, Mertz JA, Salmeron A, Dakle PJ, Mele D, Cote A, Jayaram H, Setser JW, Poy F, Hatzivassiliou G, DeAlmeida-Nagata D, Sandy P, Hatton C, Romero FA, Chiang E, Reimer T, Crawford T, Pardo E, Watson VG, Tsui V, Cochran AG, Zawadzke L, Harmange JC, Audia JE, Bryant BM, Cummings RT, Magnuson SR, Grogan JL, Bellon SF, Albrecht BK, Sims RJ 3rd, Lora JM.	J Biol Chem. 2016 Jun 17;291(25):13014-27.
GSK6853, a Chemical Probe for Inhibition of the BRPF1 Bromodomain.	Bamborough P, Barnett HA, Becher I, Bird MJ, Chung CW, Craggs PD, Demont EH, Diallo H, Fallon DJ, Gordon LJ, Grandi P, Hobbs CI, Hooper-Greenhill E, Jones EJ, Law RP, Le Gall A, Lugo D, Michon AM, Mitchell DJ, Prinjha RK, Sheppard RJ, Watson AJ, Watson RJ.	ACS Med Chem Lett. 2016 May 9;7(6):552-7.
Fast and high resolution single-cell BRET imaging.	Goyet E, Bouquier N, Ollendorff V, Perroy J.	Sci Rep. 2016 Jun 15;6:28231.
The TIP60 Complex Is a Conserved Coactivator of HIF1A.	Perez-Perri JI, Dengler VL, Audetat KA, Pandey A, Bonner EA, Urh M, Mendez J, Daniels DL, Wappner P, Galbraith MD, Espinosa JM.	Cell Rep. 2016 Jun 28;16(1):37-47.
Determination of GLUT1 Oligomerization Parameters using Bioluminescent Förster Resonance Energy Transfer.	Looyenga B, VanOpstall C, Lee Z, Bell J, Lodge E, Wrobel K, Arnoys E, Louters L.	Sci Rep. 2016 Jun 30;6:29130.
Mutations of Vasopressin Receptor 2 Including Novel L312S Have Differential Effects on Trafficking.	Tiulpakov A, White CW, Abhayawardana RS, See HB, Chan AS, Seeber RM, Heng JI, Dedov I, Pavlos NJ, Pfleger KD.	Mol Endocrinol. 2016 Aug;30(8):889-904.
Sensitivity and engineered resistance of myeloid leukemia cells to BRD9 inhibition.	Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G, McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, Suzuki Y, Koegl M, Vakoc CR.	Nat Chem Biol. 2016 Sep;12(9):672-9.
Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the human β1-adrenoceptor expressed in HEK-293 cells.	Soave M, Stoddart LA, Brown A, Woolard J, Hill SJ.	Pharmacol Res Perspect. 2016 Aug 8;4(5):e00250.
1,3-Dimethyl Benzimidazolones Are Potent, Selective Inhibitors of the BRPF1 Bromodomain.	Demont EH, Bamborough P, Chung CW, Craggs PD, Fallon D, Gordon LJ, Grandi P, Hobbs CI, Hussain J, Jones EJ, Le Gall A, Michon AM, Mitchell DJ, Prinjha RK, Roberts AD, Sheppard RJ, Watson RJ.	ACS Med Chem Lett. 2014 Sep 10;5(11):1190-5.
Discovery of I-BRD9, a Selective Cell Active Chemical Probe for Bromodomain Containing Protein 9 Inhibition.	Theodoulou NH, Bamborough P, Bannister AJ, Becher I, Bit RA, Che KH, Chung CW, Dittmann A, Drewes G, Drewry DH, Gordon L, Grandi P, Leveridge M, Lindon M, Michon AM, Molnar J, Robson SC, Tomkinson NC, Kouzarides T, Prinjha RK, Humphreys PG.	J Med Chem. 2016 Feb 25;59(4):1425-39.
AZD5153: A Novel Bivalent BET Bromodomain Inhibitor Highly Active against Hematologic Malignancies.	Rhyasen GW, Hattersley MM, Yao Y, Dulak A, Wang W, Petteruti P, Dale IL, Boiko S, Cheung T, Zhang J, Wen S, Castriotta L, Lawson D, Collins M, Bao L, Ahdesmaki MJ, Walker G, O’Connor G, Yeh TC, Rabow AA, Dry JR, Reimer C, Lyne P, Mills GB, Fawell SE, Waring MJ, Zinda M, Clark E, Chen H.	Mol Cancer Ther. 2016 Nov;15(11):2563-2574.
Potent and selective bivalent inhibitors of BET bromodomains.	Waring MJ, Chen H, Rabow AA, Walker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers MB, Saif S, Stratton N, Svergun DI, Wang W, Whittaker D, Wilson DM, Yao Y.	Nat Chem Biol. 2016 Dec;12(12):1097-1104.
Enhancing Specific Disruption of Intracellular Protein Complexes by Hydrocarbon Stapled Peptides Using Lipid Based Delivery.	Thean D, Ebo JS, Luxton T, Lee XC, Yuen TY, Ferrer FJ, Johannes CW, Lane DP, Brown CJ.	Sci Rep. 2017 May 11;7(1):1763.
The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein	Miyakawa K, Nishi M, Matsunaga S, Okayama A, Anraku M, Kudoh A, Hirano H, Kimura H, Morikawa Y, Yamamoto N, Ono A, Ryo A.	Nature Communications 2017 Jan 30;8:14259
PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.	Moriya C, Taniguchi H, Nagatoishi S, Igarashi H, Tsumoto K, Imai K	Cancer Sci. 2018 Feb;109(2):373-383
Visualizing Ligand Binding to a GPCR In Vivo Using NanoBRET	Alcobia DC, Ziegler AI, Kondrashov A, Comeo E, Mistry S, Kellam B, Chang 2, Woolard J, Hill SJ, Sloan EK	iScience 2018 Aug 31;6:280-288. doi: 10.1016/j.isci.2018.08.006.

NanoBiT

※ 論文タイトルをクリックするとPubMedに移動します

タイトル	著者	ジャーナル名
NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells	Dixon AS, Schwinn MK, Hall MP, Zimmerman K, Otto P, Lubben TH, Butler BL, Binkowski BF, Machleidt T, Kirkland TA, Wood MG, Eggers CT, Encell LP, Wood KV.	ACS Chem Biol. 2016 Feb 19;11(2):400-8.
Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells	Zhao J, Nelson TJ, Vu Q, Truong T, Stains CI.	ACS Chem Biol. 2016 Jan 15;11(1):132-8.
SOD1 dimerization monitoring using a novel split NanoLuc, NanoBit	Oh-Hashi K, Hirata Y, Kiuchi K.	Cell Biochem Funct. 2016 Oct;34(7):497-504.
Detection and Activity Profiling of Synthetic Cannabinoids and Their Metabolites with a Newly Developed Bioassay	Rouault AAJ, Lee AA, Sebag JA.	Biochim Biophys Acta. 2017 Dec;1864(12):2322-2329.
Development of a bimolecular luminescence complementation assay for RGS: G protein interactions in cells	Bodle CR, Hayes MP, O’Brien JB, Roman DL.	Anal Biochem. 2017 Apr 1;522:10-17.
The prohibitin-repressive interaction with E2F1 is rapidly inhibited by androgen signalling in prostate cancer cells	Koushyar S, Economides G, Zaat S, Jiang W, Bevan CL, Dart DA.	Oncogenesis. 2017 May 15;6(5):e333.
An enzyme-mediated protein-fragment complementation assay for substrate screening of sortase A	Li N, Yu Z, Ji Q, Sun J, Liu X, Du M, Zhang W.	Biochem Biophys Res Commun. 2017 Apr 29;486(2):257-263.
TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole	Oberstadt M, Stieler J, Simpong DL, Römuß U, Urban N, Schaefer M, Arendt T, Holzer M.	Sci Rep. 2018 Feb 2;8(1):2248.
Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration	Matsumoto T, Matsukawa K, Watanabe N, Kishino Y, Kunugi H, Ihara R, Wakabayashi T, Hashimoto T, Iwatsubo T.	Hum Mol Genet. 2018 Apr 15;27(8):1353-1365.
A Split-Luciferase-Based Trimer Formation Assay as a High-throughput Screening Platform for Therapeutics in Alport Syndrome	Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico M, Kai H.	Cell Chem Biol. 2018 May 17;25(5):634-643.e4.
Application of NanoBiT for Monitoring Dimerization of the Null Hong Kong Variant of α-1-Antitrypsin, NHK, in Living Cells.	Norisada J, Fujimura K, Amaya F, Kohno H, Hirata Y, Oh-Hashi K,	Mol Biotechnol. 2018 Jun 6. doi: 10.1007/s12033-018-0092-5. [Epub ahead of print]